MicroFlow NMR

MicroFlow NMR

Acquire High Sensitivity Spectra Quickly and Easily
MicroFlow NMR combines feature rich high sensitivity with supreme ease of use. Operating at the capillary-scale will save time and money while enhancing your sensitivity. For increased functionality at a remarkable value, nothing else compares.

  • Save sample preparation time and cost.

    Spend less time in the field collecting raw material.

  • Get Spectra From a Few Micrograms

    LCapNMR Probes excel in mass-limited applications.

  • Flexible Sample Loading Options

    Load from a simple syringe or automate with a liquid handlers.

  • Easy Sample Recovery

    Push the sample in. Park for hours. Then push it out. Recollect.

  • Compatible with 200 MHz up to 800 MHz

    CapNMR works with Agilent, Bruker, JEOL and Oxford magnets.

  • Use all standard NMR experiments.

    Run 1H, 13C, COSY, HSQC, HMBC, NOESY.  Strong z-gredient.

One-Minute NMR

Automation for Medicinal Chemists

Find New Molecular Entities

Find New Molecular Entities

Run NMR on Your Existing LC-MS Fractions



Log in Samples Rapidly from Any Browser, Table or Phone


Log in Samples Rapidly from Any Browser, Table or Phone

1m - Single Page

Net More Results


Featured Speaker

Dr. Dave Detlefsen, PANIC  Talk, February, 2014


Reference Customer

Contract Research Lab

NMR Structure Elucidation


The Protasis CapNMR DPC Probe has dual 10uL flowcells and so can run two NMR samples at the same time (by interleaving). This unique, patented innovation was developed by Protasis under Pfizer’s Strategic Alliance program and is deployed at Pfizer MedChem labs in Groton, CT and La Jolla, CA.


Collect Less. Get Published Faster!

Get NMR spectra from a few micrograms of your precious sample.


Go to our Web Store to order a free sample kit!

MicroFlow NMR

Acquire High Sensitivity Spectra Quickly and Easily

MicroFlow NMR Probes

Sensitivity. Simplicity.  Economy.


Factors most important to me for data collection and research publication

  • Probe where < 10μL and < 25μg of material is sufficient for NMR analysis
  • Probe that can be easily installed/shimmed by my NMR facility manager and used by my students
  • Probe where sample introduction and recovery don’t require that I become a fluidics expert
  • Probe that doesn’t require extensive maintenance and service
  • Probe where the S/N is preserved even if the salt concentration is high
  • Probe where I do not need an NMR tube
  • Flowprobe with minimal solvent rinse requirements; < 50μL yields < 1% carryover
  • Probe that accommodates a wide range of solvents, buffers, and pH conditions
  • NMR measurement solution that enables research, not capital equipment, to dominate my budget
  • Probe that is easily repaired in a timely manner and for a reasonable fee if damaged
  • Ability to completely recover my samples into the original vial.

Customer Questions

Probe Performance: How can I achieve high mass sensitivity with low solvent volume requirements?

The Protasis/MRM CapNMR probe is designed throughout as a capillary-scale device. With a total internal volume of 7μL and a flow cell volume of 4.5μL (active volume is 1.5 μL), it’s the smallest volume NMR probe on the market. The tremendous mass sensitivity is sufficient to obtain a 1H S/N > 10:1 from 1μg of material in only a few minutes. The probe is easy to use, requiring adjustment of only a few shims when first inserted in the magnet, and after solvent changes, while virtually no shimming is necessary for samples in the same solvent.  Highly conductive (e.g. salty) samples are no problem for this probe – less than a 10% S/N penalty for salt concentrations as high as 0.5M.

Sample Management: What are my options?

Sample management options range from the very simple, practical, and economical approach of direct syringe injection to more elaborate techniques involving sample vials, 96 well plate format in automation and capillary chromatographic hyphenations. While the more involved techniques offer a greater range of functionalities, the simple approaches to sample introduction shouldn’t be dismissed.

Sequoia Sciences, a company dedicated to drug lead generation from natural product libraries has analyzed well over 300 natural products using the CapNMR probe.

Cost Savings: Can I save on deuterated solvents? Do I still need tubes?

“’Tubeless NMR’ enables us to rapidly, accurately, and economically characterize our samples in a manner that fits our business strategy” says Sequoia’s Mark O’Neil-Johnson. “Over the past year, we have totally eliminated NMR tubes from our NMR acquisition process. Not only has this resulted in savings of time and money, butmore importantly, it has enabled us to move into a regime where microliter volume analysis is standard.” “The CapNMR probe has had a tremendous impact on our business” says Gary Eldridge, President of Sequoia. “We have grown to depend on the capabilities that this technology brings to our operation and I can unequivocally say that we would not be as far along with our business success as we are today with any other available NMR probetechnology.”

Protasis/MRM offers high throughput systems for NMR analysis. But we also offer options for easy, manual loading that can be accomplished with a minimal knowledge of sample handling techniques. We offer application note NMR-10 that fully describes the manual method of sample loading. You can request this note at info@protasis.com.

Press Release
Find New Molecular Entities

Find New Molecular Entities

More Information

MicroFlow NMR Documents

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White Paper – Less is More

The combination of sensitive capillary NMR probes, fast liquid handlers and advanced heteronuclear methods is allowing NMR to take its place alongside LC-MS in life science research. Using only micrograms of sample, MicroFlow NMR can provide definitive verification of structure and quantitation of sample amount and purity for routine characterization of new or existing molecular […]