One-Minute NMR Platform


One-Minute NMR Overview

Now you can obtain the highest quality NMR spectra automatically with less sample in less time:

  • LEAP & Tower Side 2 HQHighest mass sensitivity (micrograms)
  • One minute fluidic cycle time
  • One minute proton spectra
  • 2D spectra in a few hours
  • One-Minute NMR Software
  • Web-based with the same browser interface for Agilent, Bruker, Varian INOVA and JEOL
  • Load from standard laboratory consumables;
    • low volume vials
    • 96-well plates
    • 384-well plates, etc.
  • Downsize to MicroFlow NMR and save on sample utilization, reagent cost, tube cost and magnet time

Who uses NMR Automation?

  • Mass-limited researchers building full libraries of 1D and 2D spectra
  • Multi-user facilities that want improved NMR productivity
  • Biofluids analysts need higher throughout for serum and urine
  • Facility managers wanting to breathe new life into existing 400 MHz NMR systems
  • Anyone that wants automated use standard microplates or vials

We believe that end-user collaboration is key to creation of quality products that deliver the results you need. 

The One-Minute NMR system was designed by a team of NMR spectroscopists, software developers and fluidic engineers.  Participating NMR users helped out by specifying their needs and providing feedback as development began. The resulting modular, expandable Discovery Platform can keep up with your  sample demand today and can evolve to meet your laboratory’s changing needs and increasing workloads.  One-Minute NMR delivers advanced features across the discovery and development value chain, from natural product chemists to biofluids analysis to trace impurity identification.  Every chemist and their managers will appreciate the productivity boost and convenience of walk-away, unattended operation, with special support for overnight samples.  When you consider the reduced sample prep for new samples, reduced sample utilization from existing libraries, reduced reagent and solvent consumption, elimination of the cost and climbing of tubes. and improved magnet utilization due to faster spectral acquisition, this is a system that easily justifies itself in any NMR facility with multiple users or many samples.

Protasis Discovery Platforms deliver amplified performance in software and hardware, applied to real-world customers and samples.


One-Minute NMR Requirements

Operating System

Windows XP Professional (Legacy Support for Existing Customers)

Windows 7, 8 or 8.1


4GB (minimum)

Hard Drive

1TB (typical installation)


Windows Professional is required. .NET Framework and SQ Server run-time are used.

Compatible NMR Operating Systems


Windows (Bruker)

Compatible Spectrometers






Internet Explorer 8 should be used for Windows XP systems.

Internet access is required for the installation.


MicroFlow NMR Features List

Features List


Advanced features make MicroFlow NMR a
superior alternative in many applications
to both conventional and cryo-cooled NMR probes.

CapNMR probes provide outstanding value
that will give you exceptional results and
pay for itself in a few months of normal use.

You can investigate further on our feature tour.



Performance, High Sensitivity


Get Spectra From A few Micrograms of Sample

Low Power Allows Broadband Spectra

High Resolution

High Fill Factor and High Observe Factor

Low Noise / Clean Baselines

High Signal-to-Solvent Ratio

Excellent Salt Tolerance

Rapid Recovery from Steep Solvent Gradients

High Magnetic Field Gradients

Excellent Multi-Nuclear Sensitivity

Excellent Thermal Stability

Efficient Sample Delivery and Recovery


Simple and Effective


Sensible Sensitivity

Easy Shimming

Easy Tuning

Use All Your Standard NMR Methods

Sample Management Made Simple

Compatible with Varian, Bruker and JEOL

Easy Sample Recovery

Simple, Smooth Flowpath

Compatible w/ 300 MHz – 800 MHz Field Strengths

Excellent Solvent Compatibility


A Technology Boost That Pays for Itself


More Information, Less Sample

Consume Less Solvent

Affordable High Sensitivity

Maximize Magnet Utilization

Eliminate Tube Costs

Use Deuterated Solvents Exclusively

Lower Disposal Costs


Get the Spectra Now–Get Published Faster


Walk-up NMR

Rapid Probe Re-Installation

Fast Shimming with Presets

Fast Syringe Injection

Rapid Sample Loading up to 5 Meters Away

Up to 100x Faster than a Conventional 5mm Probe

Reduce Time to Acquire Full Set of 1D & 2D Spectra

No Waiting for Sample Recovery

Rapid Rinsing for Undetectable Carryover

Rapid Probe Removal and Storage


Flow-Through Reliability


Flexible, Precise Injections

Replaceable Filters Protect Flowpath

Backpressure Monitoring


Comprehensive Support


On-Site Installation
On-Site Training
Detailed Documentation
90-Day Free Telephone Support

Online Help Desk
FREE Technical Support by Email
Premium Telephone Support
Web-Based Training
per Incident Repairs OR Umbrella Coverage
Software Updates
Consumables Kit

Your answer to Affordable High Sensitivity. MicroFlow NMR


White Paper – Less is More

mathsymbolslessthanbnw-150x150The combination of sensitive capillary NMR probes, fast liquid handlers and advanced heteronuclear methods is allowing NMR to take its place alongside LC-MS in life science research. Using only micrograms of sample, MicroFlow NMR can provide definitive verification of structure and quantitation of sample amount and purity for routine characterization of new or existing molecular libraries. Read the white paper to learn about microil sensitivity, simplicity and economy!




BU-CMLD Loads 54-Vial Tray

Open-Access NMR


“The walk-up spectrometers are accounted in 10-minute increments and can be used reserved for blocks of time ranging from 10 minutes to 24 hours depending on the time of day and whether the usage is on a weekend or weekday. To gain access to these instruments, please Request a xxxx CIC Account. After we have evaluated your needs, CIC staff will create your accounts and schedule training sessions as appropriate.”


  1. Log in your sample from any browser, tablet or smartphone.
  2. Bring the sample to the NMR system and place it in the reserved tray location.
  3.  Click the Run button. Walk away.  Your results will be waiting in your Inbox.



SMASH 2012

Protasis hosted a table at this year’s Small Molecule NMR (SMASH) Conference on September 9-12 in Providence, RI. We set up a One-Minute NMR system and ran it through its sample loading paces. The conference was held jointly with CoSMoS, the Society for Small Molecule Science with about 300 total attendees. Protasis showed two joint posters with our customer collaborators on CryoFlow NMR:

Poster #49
Improvements in Flow-Injection NMR as a Tool for High-Throughput Sample Analysis
Paul Krolikowski (1), L. Steven Hollis(1), Roger Kautz (2) and David Strand (3)

1.  Molecular Structure, Amgen Inc., Cambridge, MA, USA
2.  Barnett Institute at Northeastern University, Boston, MA, USA
3.  Protasis Corporation, Marlboro, MA, USA

Recently, dramatic improvements in S/N have been obtained using customized flow-NMR instrumentation by incorporating a small volume flow-cell (10-60 uL) with a high-sensitivity 600 MHz Bruker 5-mm TCI-cryoprobe and a Protasis sample delivery system. The large sensitivity gain provided by cryoprobe technology (as much as 20X increase compared to a micro-coil 500 MHz RT flow-probe) has dramatically reduced data collection times to a point where routine 1H and g-HSQCdept spectra can be collected on small samples (25 uL at 20 mM) in under 10 minutes. These hardware improvements have allowed implementation of rapid quantitative-NMR analysis for HTPP (High Throughput Purification Process) samples using Digital-Eretic referencing as an integral calibration technique. In addition, new flow-cell inserts have been designed which easily can be interchanged to alter sample volume or switched from flow to tube based operations in just a few minutes with minimal setup time.
The concept of tailoring the flow-cell volume to match that of the intended sample stream (with an active volume of 10, 30 or 60 uL) using a standard Bruker pass-through cryoprobe, will be discussed as part of our continuing effort to improve NMR sampling efficiency. The Protasis sample delivery station and operating system (One-Minute NMR) provides a flexible and convenient platform for submitting samples in 96-well plate format, or as single samples in walk-up mode. Automated data processing using ACD Automation Server provides a reliable solution for archiving and distributing the large amounts of processed data that are generated from plate-base HTPP submissions. In addition, segmented-flow NMR methods (SFA), using a susceptibility matched push-solvent (FC-43) to limit sample dispersion in the flow cell, are also being investigated in an effort to improve S/N with mass-limited samples. The initial results of these studies, which show up to a two-fold improvement in S/N vs. single-solvent push techniques, will be presented.

Poster #21
Segmented Flow Into  LC Probes Double Flow-NMR Sensitivity and Throughput
Roger Kautz (1), David Strand (2), and Steve Hollis (3)

1.  Barnett Institute at Northeastern University, Boston, MA
2.  Protasis Corporation, Marlboro, MA
3.  Amgen Corporation, Cambridge, MA

* Maintains uniform original concentration, for ligand-binding or qNMR studies.
* Confines sample in NMR coil volume, for optimal sensitivity.
* Improves throughput by 10 min/sample; reduces solvent consumption 5-fold.
* Applicable to cryo-flow for trace sensitivity, or old LC probe for high-throughput.

Flow-injection NMR (FIA-NMR) has proven valuable for acquiring spectra directly from vials or 96-well plates, avoiding the time and expense of preparing samples in tubes, and specialized automation to load tubes. Extending our previous work in segmented flow loading of microcoil probes (“microdroplet NMR”) [1], we here show how segmented flow (SFA) loading solves several problems seen with conventional flow-NMR into LC probes (60 uL or larger).

Segmented flow loads samples without dispersion or dilution — the sample is transported like a drop of water in oil. The flow system is filled with an immiscible fluid (fluorocarbon FC43) so the sample moves as a droplet or, in narrow tubing, as an elongated plug with a sharp boundary and with uniform (undiluted) concentration throughout. FC43 has magnetic susceptibility matched to D2O, and functions as “Shigemi tubes for a flow system” to confine the entire sample within the observed volume. To implement segmented flow, use of a fluorinated fluid in Teflon tubing is ideal; fluoro-silanized glass surfaces also work well. The data presented were obtained by using a stock commercial flow-NMR platform (Protasis One-Minute NMR) with a Teflon sample loop and transfer line. SFA and FIA were compared in in video of injected dye, and in on-flow and stopped-flow NMR spectra of a standard (20 mg/mL caffeine in D2O).

Results: SFA loading was explored in a cryo-flow system with a 60 uL nominal NMR observed volume (120 uL flowcell volume; 150 uL dead vol to center of V-obs). SFA loading 70 uL of the standard gave twice (2x) the NMR signal intensity as FIA loading; which was 95% the signal intensity of a flowcell filled with the standard. A 50 uL sample could be shimmed to the same lineshape as 70 uL or larger, but accordingly shows only 70% of the signal strength (50 uL/70 uL = 71%). Video of dye injections by SFA and FIA provide a concrete picture. Samples loaded with SFA move into the detected volume as a discrete object, like a car into a garage; accordingly, on-flow NMR signal strength rises linearly from first light to 100% as the leading edge of sample fills the coil volume; plateaus at 100% while the coil is filled, then linearly decreases to 0 as the trailing boundary of the sample passes out of the detector region. FIA samples, in contrast, arrive like the hot water at a distant tap: signal intensity increases more slowly with a slope projecting to 100% after 0.7 dead volumes, but cresting and tailing for smaller samples. Parabolic flow in the flowcell produced large concentration gradients both radially and axially, reflected in the lineshape (30 Hz) of the caffeine methyl resonances, which are concentration-sensitive. When flushing samples, the tailing in FIA required 300 uL (nearly 3 flowcell volumes) to bring residual signal below 2%.

Applications: Loading samples at a defined and uniform concentration enables ligand-binding or qNMR studies. Confining samples into the observed volume gives the theoretical optimum sensitivity for a probe, under unattended automation. SFA-NMR with cryoprobes gives high sensitivity; an old LC probe would serve for high throughput of routine chemistry samples.

1.  Lin et al, Anal. Chem 80: 8045 (2008)

SMASH 2012 program:


Windows XP is Dead, Long Live Windows 8.1

What is end of support?
After 12 years, support for Windows XP ended April 8, 2014. There will be no more security updates or technical support for the Windows XP operating system. It is very important that customers and partners migrate to a modern operating system such as Windows 8.1. Customers moving to a modern operating system will benefit from dramatically enhanced security, broad device choice for a mobile workforce, higher user productivity, and a lower total cost of ownership through improved management capabilities.

How do I migrate off Windows XP?
Learn more about migration and deployment programs by contacting your Microsoft sales representative, Microsoft Services or your Certified Microsoft Partner. If your current PC meets the system requirements for Windows 7 or Windows 8.1, you can buy Windows 7 Professional or Windows 8.1 Pro from a local retailer or Microsoft Certified Partner. If your PC does not meet system requirements, consider purchasing a new business PC with Windows 8.1 Pro.

Potential risks of staying with Windows XP
Running Windows XP SP3 in your environment after April 8, 2014 may expose you to potential risks, such as:

Without critical Windows XP security updates, your PC may become vulnerable to harmful viruses, spyware, and other malicious software which can steal or damage your business data and information. Anti-virus software will also not be able to fully protect you once Windows XP itself is unsupported.

Businesses that are governed by regulatory obligations such as HIPAA may find that they are no longer able to satisfy compliance requirements. More information on HHS’s view on the security requirements for information systems that contain electronic protected health information (e-PHI) can be found here (HHS HIPAA FAQ – Security Rule).

Lack of Independent Software Vendor (ISV) Support:
Many software vendors will no longer support their products running on Windows XP as they are unable to receive Windows XP updates. For example, the new Office takes advantage of the modern Windows and will not run on Windows XP.

Hardware Manufacturer support:
Most PC hardware manufacturers will stop supporting Windows XP on existing and new hardware. This will also mean that drivers required to run Windows XP on new hardware may not be available.

Frequently Asked Questions

Can Windows XP still be activated after April 8, 2014?
Windows XP can still be installed and activated after end of support. Computers running Windows XP will still work but they won’t receive any Microsoft Updates or be able to leverage technical support. Activations will still be required for retail installations of Windows XP after this date as well.

Can Windows XP Mode in Windows 7 still be used in Windows XP?
Windows XP Mode follows the same support lifecycle as Windows XP, extended support will end April 8, 2014.

Will Microsoft Security Essentials be supported after April 8, 2014?
Microsoft Security Essentials will not be available for download on Windows XP after April 8, 2014. If you already have Microsoft Security Essentials installed, you will continue to receive anti-malware signature updates through July 14, 2015. However, please note that PCs running Windows XP after April 8, 2014 should not be considered protected.

Will Microsoft’s Malicious Software Removal Tool be supported after April 8, 2014?
Microsoft’s Malicious Software Removal Tool is aligned with the company’s anti-malware engines and signatures, and as such the removal tool will continue to be provided for Windows XP through July 14, 2015. However, any PC running Windows XP after April 8, 2014 should not be considered protected as there will be no security updates for the Windows XP operating system.

Will System Center, Windows Intune, and Microsoft Deployment Toolkit still support Windows XP?
While customers may continue to use System Center, Windows Intune, and the Microsoft Deployment Toolkit to manage and deploy Windows XP past April 8, 2014, those products will no longer support Windows XP, and any technical issues which may arise will not be addressed.

What about Windows XP Embedded?
See the Windows Embedded product lifecycle page and Microsoft Support for more information on Windows XP Embedded lifecycles.

Will existing updates still be available via Windows Update after April 8, 2014?
Yes, all existing Windows XP updates and fixes will still be available via Windows Update and WSUS.

Will Internet Explorer 8 still be supported on Windows XP?
As a component of Windows, Internet Explorer follows the support lifecycle of the Windows operating system on which it is installed on. More information is available at Microsoft Support.

Which machines will receive the Windows XP End of Support notification?
The notification will be sent to users of Windows XP Home and Windows XP Professional who have elected to receive updates via Windows Update. Users in organizations using Windows Server Update Services (WSUS), System Center Configuration Manager, or Windows Intune will not receive the Windows XP end of support notification.